Abstract

背景+問題：The C2H2-zinc finger protein (C2H2-ZFP) is essential for the regulation of plant development and widely responsive to diverse stresses including drought, cold and salt stress, further affecting the late flavonoid accumulation in higher plants. Tea is known as a popular beverage worldwide and its quality is greatly dependent on the physiological status and growing environment of the tea plant. To date, the understanding of C2H2-ZFP gene family in Camellia sinensis [L.] O. Kuntze is not yet available. 

結(jié)果1：In the present study, 134 CsC2H2-ZFP genes were identified and randomly distributed on 15 chromosomes. The CsC2H2-ZFP gene family was classified into four clades and gene structures and motif compositions of CsC2H2-ZFPs were similar within the same clade. Segmental duplication and negative selection were the main forces driving the expansion of the CsC2H2-ZFP gene family. 

結(jié)果2：Expression patterns suggested that CsC2H2-ZFPs were responsive to different stresses including drought, salt, cold and methyl jasmonate (MeJA) treatment. Specially, several C2H2-ZFPs showed a significant correlation with the catechins content and responded to the MeJA treatment, which might contribute to the tea quality and specialized astringent taste.

結(jié)論：This study will lay the foundations for further research of C2H2-type zinc finger proteins on the stress responses and quality-related metabolites accumulation in C. sinensis.

摘  要

C2H2-鋅指蛋白（C2H2-ZFP）是調(diào)節(jié)植物發(fā)育必不可少的，并且廣泛響應(yīng)包括干旱，寒冷和鹽脅迫在內(nèi)的各種脅迫，進一步影響了高等植物中類黃酮積累。茶在世界范圍內(nèi)被稱為流行飲料，其品質(zhì)在很大程度上取決于茶樹的生理狀況和生長環(huán)境。迄今為止，尚無對茶樹中的C2H2-ZFP基因家族的了解。在本研究中，鑒定了134個CsC2H2-ZFP基因，并將其隨機分布在15條染色體上。CsC2H2-ZFP基因家族分為四個進化枝，CsC2H2-ZFPs的基因結(jié)構(gòu)和基序組成在同一進化枝中相似。節(jié)段重復(fù)和負選擇是驅(qū)動CsC2H2-ZFP基因家族擴展的主要力量。表達模式表明CsC2H2-ZFPs對不同的脅迫有響應(yīng)，包括干旱，鹽，冷和茉莉酸甲酯（MeJA）處理。特別地，幾種C2H2-ZFP與兒茶素含量呈顯著相關(guān)性，并對MeJA處理有反應(yīng)，這可能有助于提高茶的質(zhì)量和特有的澀味。這項研究將為進一步研究C2H2型鋅指蛋白在茶樹的應(yīng)激反應(yīng)和與質(zhì)量相關(guān)的代謝物積累方面奠定基礎(chǔ)。